Nitsch Medium
The medium defined by Nitsch was used in the production of haploid plants of various species of Nicotiana raised from pollen grains. In this procedure, pollen that were still uninucleate were isolated and then cultured in vitro. Some pollen grains proliferate into embryo-like structures that develop in stages similar to those of zygotic embryos. The plantlets matured and flowered profusely, but did not set seed.
貨號：N0224.0050 Nitsch Medium含維生素 規格：50升
Total concentration Micro and Macro elements including vitamins: 2178.74 mg/l.
貨號：N0223.0050 Nitsch Medium不含維生素 規格：50升
Total concentration Micro and Macro elements not including vitamins: 2070.19 mg/l.
|
Macro Elements
|
mg/l
|
mM
|
|
CaCl2
|
166.00
|
1.50
|
|
KH2PO4
|
68.00
|
0.50
|
|
KNO3
|
950.00
|
9.40
|
|
MgSO4
|
90.27
|
0.75
|
|
NH4NO3
|
720.00
|
9.00
|
|
Micro Elements
|
mg/l
|
μM
|
|
CuSO4.5H2O
|
0.025
|
0.10
|
|
FeNaEDTA
|
36.70
|
100.00
|
|
H3BO3
|
10.00
|
161.73
|
|
MnSO4.H2O
|
18.94
|
111.94
|
|
Na2MnO4.2H2O
|
0.25
|
1.03
|
|
ZnSO4.7H2O
|
10.00
|
34.78
|
|
Vitamins
|
mg/l
|
μM
|
|
Biotin
|
0.05
|
0.21
|
|
Folic acid
|
0.50
|
1.13
|
|
Glycine
|
2.00
|
26.64
|
|
myo-Inositol
|
100.00
|
554.94
|
|
Nicotinic acid
|
5.00
|
40.62
|
|
Pyridoxine HCl
|
0.50
|
2.43
|
|
Thiamine HCl
|
0.50
|
1.48
|
Nitsch J.P. and Nitsch C., Haploid plants from pollen grains, Science 169, 85 (1969). Nitsch J.P., Experimental androgenesis in Nicotiana, Phytomorphology 19, 389 (1969).